The LIFA is a camera-based FLIM system that can be easily combined with the HSi hyperspectral unit of Gooch & Housego. Acquiring lifetimes at a range of wavelengths opens up the possibility to spectrally resolve multi-component lifetime distributions into their lifetime components.
Moreover spectrally resolved FLIM allows one to track both the donor and acceptor molecules in FRET experiments, and enables the observation of a range of spectroscopic changes invisible to a standard FLIM system. The addition of polarizers further enables the detailed anisotropy characterisation required in diffusion studies.
The widefield configuration includes the new Multi-LED modulated light source. Its high-power LEDs can be modulated in a broad frequency range, resulting in good lifetime sensitivity and high accuracy.
The LIFA is compatible with Leica®, Nikon®, Olympus®, TILL® and Zeiss® fluorescence microscopes.
Fast FLIM, up to two lifetime images per second
Non-phototoxic illumination offered by the Multi-LED (NEW)
Lifetime sensitivity 0-300 ns
Lifetime accuracy 30 ps r.m.s.
High quantum efficiency with the optional Gen III GaAs intensifier
Hardware features HSi
For the HSi hardware features please follow this link.
Full control of the HSi unit from LI-FLIM
FRET efficiency mapping
Polar (Phasor) plot inspection and separation of multiple lifetimes
Export to Metamorph, ImageJ, Matlab
Images can be exported as BMP, TIFF
Statistics and histograms can be exported as MS Excel
LI-FLIM software features:
Lambert Instruments provides the following LIFA models, which cover a wide range in spectral sensitivity and in lifetime sensitivity. Each model is based on a specific high-resolution Gen II or Gen III image intensifier.
LIFA GenII S20
LIFA GenII S25
LIFA GenIII GaAs
LIFA X GenII S20
LIFA X GenII S25
LIFA X GenIII GaAs
LIFA P GenII S20
LIFA P GenII S25
LIFA P GenII GaAs
LIFA P GenIII GaAsP
For each of the models the following graph shows the spectral sensivity versus wavelength.
Click here for more information on the operating principle of image intensifiers for lifetime imaging.