LIFA Hyperspectral

LIFA Hyperspectral
LIFA Hyperspectral

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  • Description
  • Features
  • Models
  • Options
  • Applications

  • The LIFA is a camera-based FLIM system that can be easily combined with the HSi hyperspectral unit of Gooch & Housego. Acquiring lifetimes at a range of wavelengths opens up the possibility to spectrally resolve multi-component lifetime distributions into their lifetime components.

    Moreover spectrally resolved FLIM allows one to track both the donor and acceptor molecules in FRET experiments, and enables the observation of a range of spectroscopic changes invisible to a standard FLIM system. The addition of polarizers further enables the detailed anisotropy characterisation required in diffusion studies.

    The widefield configuration includes the new Multi-LED modulated light source. Its high-power LEDs can be modulated in a broad frequency range, resulting in good lifetime sensitivity and high accuracy.

    The LIFA is compatible with Leica®, Nikon®, Olympus®, TILL® and Zeiss® fluorescence microscopes.

    lifa button new

  • Hardware features

    • Fast FLIM, up to two lifetime images per second
    • Non-phototoxic illumination offered by the Multi-LED (NEW)
    • Lifetime sensitivity 0-300 ns
    • Lifetime accuracy 30 ps r.m.s.
    • High quantum efficiency with the optional Gen III GaAs intensifier

    Hardware features HSi

    For the HSi hardware features please follow this link.

    Software features

    • Full control of the HSi unit from LI-FLIM
    • FRET efficiency mapping
    • Polar (Phasor) plot inspection and separation of multiple lifetimes
    • Export to Metamorph, ImageJ, Matlab
    • Images can be exported as BMP, TIFF
    • Statistics and histograms can be exported as MS Excel

    LI-FLIM software features:

  • Lambert Instruments provides the following LIFA models, which cover a wide range in spectral sensitivity and in lifetime sensitivity. Each model is based on a specific high-resolution Gen II or Gen III image intensifier.

    Model Photocathode Modulation frequency Lifetime sensitivity
    LIFA GenII S20 S20 1-120MHz 0-300ns
    LIFA GenII S25 S25 1-120MHz 0-300ns
    LIFA GenIII GaAs GaAs 1-120MHz 0-300ns
    LIFA X GenII S20 S20 0-100kHz, 1MHz-120MHz 0ns-1ms
    LIFA X GenII S25 S25 0-100kHz, 1MHz-120MHz 0ns-1ms
    LIFA X GenIII GaAs GaAs 0-100kHz, 1MHz-120MHz 0ns-1ms
    LIFA P GenII S20 S20 0-100kHz 100ns-1ms
    LIFA P GenII S25 S25 0-100kHz 100ns-1ms
    LIFA P GenII GaAs GaAs 0-100kHz 100ns-1ms
    LIFA P GenIII GaAsP GaAsP 0-100kHz 100ns-1ms

    For each of the models the following graph shows the spectral sensivity versus wavelength.

    photocathodes flim

    Click here for more information on the operating principle of image intensifiers for lifetime imaging.

  • Support for automated microscopes

    Nikon Ti Eclipse.

    Support for XYZ stages

  • The LIFA has the following applications:

    • Molecular interactions
    • Protein conformation
    • Biosensors
    • Oxygen concentration imaging in cells and tissue
    • NADH / FAD fluorescence dynamics
    • Viscosity imaging
    • Membrane dynamics
    • Membrane trafficking
    • LED inspection
    • Crude oil characterisation