LIFA Widefield

LIFA Widefield
LIFA Widefield
LIFA Widefield

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  • Description
  • Features
  • Models
  • Options
  • Applications

  • The LIFA - Lambert Instruments Fluorescence lifetime Attachment - is a camera-based fast FLIM system that operates in the frequency domain and is compatible with Leica®, Nikon®, Olympus®, TILL® and Zeiss® fluorescence microscopes.

    The well-established homodyne detection technology together with the massive parallelism of the state-of-the-art intensified camera allows near instantaneous acquisition of full field lifetime images. The widefield system includes a Multi-LED modulated light source. Its high-power LEDs can be modulated in a broad frequency range, resulting in good lifetime sensitivity and high accuracy. The components of the LIFA are the TRiCAM modulated ICCD camera, a dual signal generator, and the LI-FLIM software package.

    The LIFA is easy to install - within the hour - and very easy to operate. The LIFA is in addition well suited for high-content screening applications. The LIFA system has been judged "easy and highly quantitative" for a.o. FLIM-FRET studies.

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  • Hardware features

    • Fast FLIM, up to two lifetime images per second
    • Non-phototoxic illumination offered by the Multi-LED (NEW)
    • Lifetime sensitivity 0-300 ns
    • Lifetime accuracy 30 ps r.m.s.
    • High quantum efficiency with the Gen III GaAs image intensifier (Optional)

    Software features

    • FRET efficiency mapping
    • Time-lapses
    • Multi-frequency acquisition & analysis for separation of multiple lifetimes
    • Polar (Phasor) plot inspection and separation of multiple lifetimes
    • Easy integration into specialized image analysis pipelines through ActiveX
    • Export to Metamorph, ImageJ, Matlab
    • Images can be exported as BMP, TIFF
    • Statistics and histograms can be exported to MS Excel

    LI-FLIM software features:

  • Lambert Instruments provides the following LIFA models, which cover a wide range in spectral sensitivity and in lifetime sensitivity. Each model is based on a specific high-resolution Gen II or Gen III image intensifier.

    Model Photocathode Modulation frequency Lifetime sensitivity
    LIFA GenII S20 S20 1-120MHz 0-300ns
    LIFA GenII S25 S25 1-120MHz 0-300ns
    LIFA GenIII GaAs GaAs 1-120MHz 0-300ns
    LIFA X GenII S20 S20 0-100kHz, 1MHz-120MHz 0ns-1ms
    LIFA X GenII S25 S25 0-100kHz, 1MHz-120MHz 0ns-1ms
    LIFA X GenIII GaAs GaAs 0-100kHz, 1MHz-120MHz 0ns-1ms
    LIFA P GenII S20 S20 0-100kHz 100ns-1ms
    LIFA P GenII S25 S25 0-100kHz 100ns-1ms
    LIFA P GenII GaAs GaAs 0-100kHz 100ns-1ms
    LIFA P GenIII GaAsP GaAsP 0-100kHz 100ns-1ms

    For each of the models the following graph shows the spectral sensivity versus wavelength.

    photocathodes flim

    Click here for more information on the operating principle of image intensifiers for lifetime imaging.

  • Support for automated microscopes

    Nikon Ti Eclipse.

    Support for XYZ stages

  • The LIFA has the following applications:

    • Molecular interactions
    • Protein conformation
    • Biosensors
    • Oxygen concentration imaging in cells and tissue
    • NADH / FAD fluorescence dynamics
    • Viscosity imaging
    • Membrane dynamics
    • Membrane trafficking
    • LED inspection
    • crude oil characterisation