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Cancer Research

Revealing Cancer's Infrastructure

Lambert Instruments has been shipping the LIFA to cancer research facilities all over the world for years. We visited Dr. Kees Jalink of the Biophysics of Cell Signaling group at the Netherlands Cancer Institute. His research group purchased the first ever LIFA to leave the labs of Lambert Instruments. Ten years later, the LIFA is still their fluorescence lifetime imaging method of choice for studying signal transduction pathways in living cells.

Diffuse Optical Tomography

The TRiCAM is a gain-modulated intensified CCD camera for Near-Infrared Diffuse Optical Tomography. It allows scientific-grade imaging of tissue properties for 3D reconstruction of chromophore concentrations in biomedical optics. Its well-established frequency-domain technology allows fast acquisition of macroscopic images at high accuracy. The TRiCAM comes with a dual signal generator and power supply and optional software for extracting the phase shift and demodulation information. Lambert Instruments also offers high modulation depth laser diodes that can be modulated across a broad frequency range for optimal sensitivity.

The Lambert Instruments TRiCAM is easy to operate and has been used in optical breast cancer screening and brain imaging.

TRiCAM Key Features

  • Highly sensitive and fast diffuse optical tomography acquisition

  • Higher quantum efficiency with the optional Gen III GaAs intensifier

  • Easy integration into biomedical imaging systems

Medical diagnosis

Biological tissues show intrinsic fluorescence lifetime imaging microscopy contrast because of the presence of autofluorescent structures like riboflavins and NADH. While autofluorescence is disadvantageous for observing tagged proteins of interest with e.g. fluorescence lifetime imaging microscopy, the exploitation of differences in the autofluorescent properties of biological tissue will increase the throughput and reliability of histopathological screening.

Autofluorescence can be used to detect molecular changes arising from diseases such as cancer and to differentiate normal cells/tissues from cancerous cells/ tissues. Namely, between normal and tumour cells differences in lifetime of normal autofluorescence have been detected. One approach is the combination of fluorescence lifetime imaging microscopy to endoscopy, whereby the endoscope is equipped with a laser. During endoscopic research, the tumour cells can be recognised and immediately burned away with the high-power laser.

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