THERE IS NO SIGNAL TO THE CAMERA

  • Is the microscope switched to the camera port?
  • Do you see light through the eyepiece when switching to eyepiece?
  • Are you using the right fluorescence filter cube?
  • Is there a shutter, neutral density (ND) filter or field stop in the light path? (the LIFA Multi-LED has a shutter, is it open?)
  • Did you press the 'FLIM' button to put the system into live mode?
  • Is the LED/laser on?

THE SIGNAL IS LOW

  • What is your MCP setting? Please test with MCP values up to around 800V.
  • Did you use a long enough exposure time (i.e. >100 milliseconds)?
  • Are you using the right fluorescence filter cube?
  • Are you using ND filters, please put the system in 'IDLE' mode and remove them, if any.

THERE IS NO LIGHT ON MY SAMPLE

  • Are you using the right excitation filter?
  • Is there a shutter, neutral density (ND) filter or field stop in the light path? (the LIFA Multi-LED has a shutter, is it open?)
  • Did you press the 'FLIM' button to put the system into live mode?
  • Is the LED/laser on?