The HiCAM Fluo is a high-speed camera for fluorescence applications. It records high resolution images at a frame rate of 1000 fps in the most challenging low-light conditions by using a cooled image intensifier. Packed into a compact aluminum enclosure, it is easy to attach the HiCAM Fluo to any fluorescence microscope.
Recording of a beating zebrafish heart at 2000 frames per second with the Lambert Instruments HiCAM on a fluorescence microscope. The blood cells were stained with a DS-red fluorophore.
monArch fluorescent voltage indicator. Video courtesy of Ed Boyden, MIT.
Applications of the HiCAM Fluo include:
High-speed fluorescence, bioluminescence and chemiluminescence detection for in-vivo imaging
Time-resolved imaging of fluids for microfluidic research
Flow visualization and velocity measurements using Particle Image Velocimetry (PIV)
Time-resolved imaging and spectroscopy using ultra-short exposures
Laser-Induced Fluorescence (LIF)
Super-slow motion combustion research for the automotive industry
Time-resolved imaging in plasma physics research
Single-photon imaging for astronomy
Researchers around the world use the HiCAM Fluo in their studies. Below is an overview of the most recent publications describing research that was done using a HiCAM Fluo. For a complete overview of applications, please refer to our applications pages.
Fiber-optically coupled intensifier
The HiCAM is a gated intensified high-speed camera. It has an integrated fiber-optically coupled image intensifier, which offers a unique combination of high speed imaging and increased light sensitivity down to single photon level. Because the HiCAM does not need high-intensity light sources, it is suitable for use in low-light level conditions like fluorescence imaging.
With its gated image intensifier, the camera's effective exposure time can be reduced. The minimum gate width of 10 ns (FWHM) increases the range of light levels at which the camera can be used. It also eliminates motion blur and enables time-resolved filtering.
The fanless design of the camera minimizes vibrations to ensure sharp images. Very low noise levels are achieved by Peltier cooling the image intensifier. Noise levels are reduced by a factor of up to 100 times as compared to uncooled intensified cameras.
The HiCAM Fluo records full-resolution images at 1000 fps. To increase the frame rate, the camera can use a smaller part of the sensor to reduce the image resolution. By doing so, it can operate at even higher frame rates.
The sensor in the HiCAM Fluo has a full resolution of 1280 x 1024 pixels. You can change the resolution settings in the software to increase the maximum frame rate or to increase the maximum recording duration.
The sensor in the HiCAM Fluo uses an electronic global shutter. This ensures that all pixels are read out at the same time to prevent rolling shutter effects. In combination with intensifier gating, exposure time can be reduced to 40 ns.
Minimum exposure time
Gating repetition rate
Photon gain (max.)
1280 x 1024 pixels
1000 fps at full resolution
1500 fps at 1200 x 720 pixels
4000 fps at 640 x 480 pixels
7500 fps at 256 x 256 pixels
Proximity-focused image intensifier*
* Our experienced sales engineers will advise you on the right image intensifier for your application.