LIFA


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LIFA


LIFA

Fluorescence lifetime imaging has never been easier

With the Lambert Instruments FLIM Attachment (LIFA), you record quantitative lifetime data in a matter of seconds. It's easy to connect our FLIM camera and light source to your microscope. And our specialized software records the images, analyzes the data instantly and presents the results visually for easy interpretation.

Features


Features


Features

 

It's fast

Record fluorescence lifetime imaging microscopy (FLIM) data in a matter of seconds. Compared to alternative methods like time-correlated single photon counting (TCSPC), the LIFA is over 100 times faster.

It's easy

Our advanced software instantly analyzes your data and presents the calculated fluorescence lifetimes visually. Recorded images are compatible with ImageJ, FIJI, Matlab and MetaMorph. Detailed statistical data can be exported to Excel worksheets.

It's compatible

The LIFA is compatible with every fluorescence microscope with a camera output. This includes fluorescence microscopes by Leica, Nikon, Olympus, TILL and Zeiss, as well as confocal microscopes and TIRF microscopes.

 

Other features

  • Non-phototoxic illumination
  • High quantum efficiency with the optional Gen III GaAs intensifier
  • Time-lapse recording mode
  • Forster Resonance Energy Transfer (FRET) efficiency mapping
  • Multi-frequency acquisition for separation of multiple lifetimes
  • Polar (Phasor) plot inspection and separation of multiple lifetimes
  • Easy integration into specialized image analysis pipelines

Compatibility

The LIFA is compatible with several types of microscopes:

  • Widefield fluorescence microscopes
  • Confocal spinning disk fluorescence microscopes
  • Total Internal Reflection Fluorescence (TIRF) microscopes
  • Hyperspectral imaging system by Gooch & Housego
  • Prior filter wheels and XY stage for multi-channel acquisition
 

Applications


Applications


Applications

 
 

User publications

Researchers around the world use the LIFA in their studies. Below is an overview of the most recent publications describing research that was done using a LIFA. For a complete overview of applications, please refer to our applications pages and our overview of selected LIFA publications for more information.

 

Featured LIFA Users

 

LIFA Models


An overview of the available LIFA models.

LIFA Models


An overview of the available LIFA models.

Models

 

 

Lifetime Imaging
 

Lifetime Range

Measurement Domain

Light Source Type

Typical Recording Duration

LIFA

Fluorescence
 

1 - 300 ns

Frequency

Modulated

< 1 s

LIFA-P

Phosphorescence
 

300 ns - 1 ms

Frequency

Modulated

< 1 s

LIFA-X

Fluorescence
Phosphorescence

1 ns - 1 ms

Frequency

Modulated

< 1 s

LIFA-TD

Fluorescence
Phosphorescence

2 ns - 1 ms

Time

Pulsed

100 s

Specifications


Specifications


Specifications

 

CAMERA SPECIFICATIONS

  • Pixel resolution: 1392 x 1040 pixels
  • Spatial resolution (min.): 19 to 26* lp/mm
  • Dynamic range: 12 bit
  • Effective pixel size: 10.3 μm
  • Equivalent background input: 0.25 μlx

* 19 lp/mm for GaAs and GaAsP photocathodes. 26 lp/mm for S20 and S25 photocathodes.

LIFETIME Specifications

  • Lifetime resolution: < 100 ps
  • Lifetime range: 0.1 ns - 1 ms
  • Framerate (max.): 2 lifetime images per second
 
lifa-configurations.png

Components

The LIFA system consists of threemain components:

LIFA: the signal generator that synchronizes the lightsource and the camera

TRiCAM: an intensified CCD camera that records the lifetime images

Light source: high-stability modulated Multi-LED or Multi-LASER

 

All LIFA components (green) and the microscope hardware are shown in the illustration on the left.

Test Sites


Test Sites


Try the LIFA at one of our test sites