LIFA-TD

Time-domain FLIM system for widefield fluorescence microscopes

 

The LIFA-TD offers a turn-key solution for fluorescence lifetime imaging microscopy and is compatible with every widefield fluorescence microscope. From recording the data to calculating the fluorescence lifetime, the entire measurement procedure is automated by our advanced software.

 

CAMERA SPECIFICATIONS

  • Pixel resolution: 1920 x 1200 pixel CMOS sensor
  • Spatial resolution (min.): 19 to 26* lp/mm
  • Dynamic range: 12 bit ADC
  • Effective pixel size: 7.6 μm
  • Equivalent background input: 0.25 μlx

 

System Specifications

  • Non-phototoxic illumination
  • High quantum efficiency with the optional Gen III GaAs image intensifier
  • Time-lapse recording mode
  • Easy integration into specialized image analysis pipelines
The LIFA-TD offers a turn-key solution for fluorescence lifetime imaging microscopy based on a gated intensified camera (ICCD/ICMOS) and is compatible with every widefield fluorescence microscope

* 19 lp/mm for GaAs and GaAsP photocathodes. 26 lp/mm for S20 and S25 photocathodes.

 

Configuration

The LIFA-TD configuration consists of a gated TRiCAM and a pulsed light source. The TRiCAM synchronizes with the light source using a TTL signal.

Our software records a series of images and automatically shifts the timing between the light pulse and the camera exposure. This image data can then be exported to popular imaging software for further analysis.