Introduction


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Introduction


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LIFA

Our LIFA software guides you through your FLIM experiments from start to finish. All Lambert Instruments hardware is integrated seamlessly so you can focus on your experiment. The software takes care of recording the FLIM data and instantly calculates the fluorescence lifetime.

Data acquisition


Data acquisition


 Data acquisition

 
 

FLIM settings

Setting up a FLIM acquisition is easy. The Live View images from the camera show you how each of the settings affects the resulting image.

Once you’ve configured the FLIM settings you click Record reference/sample to start the image acquisition. You can also record a single image by clicking Snapshot.

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Light-source control

Switching between wavelengths is done with a single click with the Lambert Instruments Multi-LED and Multi-LASER. Both are integrated seamlessly into the LIFA software.

 

Time-lapse

Follow changes in lifetime with a time-lapse. Simply enter the duration and the interval between lifetime images and see the results immediately in the image viewer and the graphs.

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Data analysis


Data analysis


Data analysis

Data analysis is easy with automatic lifetime calculation and visualization. You can overlay the lifetime as a pseudo-color on the image of your sample or use one of the built-in graphs. All graphs are updated continuously during image acquisition and you can choose between displaying one, two or four different graphs.

FLIM data courtesy of the Netherlands Cancer Institute.

FLIM data courtesy of the Netherlands Cancer Institute.

 

Lifetime overlay

Visualize the fluorescence lifetime as a pseudo-color overlay on your sample. You can use the automatic overlay settings or manually adjust the color overlay parameters.

Scatter plot

Scatter plots show how a value changes over time or as a function of another quantity. This is especially useful for illustrating changes in lifetime in a time-lapse.

The scatter plot chart in LIFA can show lifetime (phase) as a function of time, lifetime (modulation) as a function of time, or light intensity as a function of lifetime.

 

Histogram

Histograms are useful to get a quick overview of the values that are present in your sample and how they are distributed. The histogram chart in LIFA can show lifetime or light intensity.

Phasor plot

The phasor plot combines information from both phase and modulation to visualize the contribution of multiple lifetime components in your sample.

File format


File format


 File format

The Lambert Instruments file format (.fli) is compatible with Bio-Formats. This means you can open .fli files in analysis programs like ImageJ, CellProfiler and Matlab.

 
 

FIJI/ImageJ

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BioFormats

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Application Programming Interface


Application Programming Interface


LIFA API

The LIFA software offers an Application Programming Interface (API) that lets you automate many of its functionalities. This enables endless possibilities for integration with other hardware and software. The LIFA API .Net Assembly can be used in combination with many software platforms, including:

 

MATLAB

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Python

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C/C++

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Visual Basic

 

LabView

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Java

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