Versatile excitation light source for FLIM

The Lambert Instruments Multi-LED is a versatile pulsed excitation light source for fluorescence lifetime imaging microscopy in the frequency domain. The Multi-LED contains up to 4 LEDs that provide non-phototoxic illumination levels, have a low cost and a long economic lifespan.

Available wavelengths cover the range from 360 to 640 nm. The Multi-LED is part of the Lambert LIFA and LIFA-X products for fluorescence lifetime imaging microscopy/Forster Resonance Energy Transfer (FRET), and is seamlessly integrated with the LI-FLIM software. For each LED automatic control is available for LED selection, LED current and modulation frequency.





  • Molecular interactions

  • Protein conformation

  • Biosensors

  • Oxygen concentration imaging in cells and tissue

  • NADH / FAD fluorescence dynamics

  • Viscosity imaging

  • Membrane dynamics

  • Membrane trafficking

  • LED inspection

  • Crude oil characterization



  • Lifetime accuracy as part of the LIFA system less than 30 ps

  • Fast switching between individual LEDs, no realignment of the lightsource or other manual interventions needed.

  • Digital modulation up to 80-120 MHz, depending on the wavelength

  • Choice between lens-coupling and fiber-coupling to your microscope

  • Integrated USB 2.0 control

  • Compatible with all major microscope brands

  • Dimensions (l x w x h): 346 x 109 x 183 mm


Temperature stabilization

Increased temperature stabilization of the LED's is optionally available to minimize lifetime drifts during very long (many hours) time lapses.

Available wavelengths

All LEDs are high-quality modulating LEDs with a peak light intensity at wavelengths between 446 and 525 nm, 595 nm, 635 nm and 696 nm. Other wavelengths are available upon request.