Versatile pulsed excitation light source for FLIM

The Lambert Instruments Multi-LED is a versatile pulsed excitation light source for fluorescence lifetime imaging microscopy in the frequency domain. The Multi-LED contains up to 4 LEDs that provide non-phototoxic illumination levels, have a low cost and a long economic lifespan.

Available wavelengths cover the range from 360 to 640 nm. The Multi-LED is part of the Lambert LIFA and LIFA-X products for fluorescence lifetime imaging microscopy/Forster Resonance Energy Transfer (FRET), and is seamlessly integrated with the LI-FLIM software. For each LED automatic control is available for LED selection, LED current and modulation frequency.




  • Molecular interactions
  • Protein conformation
  • Biosensors
  • Oxygen concentration imaging in cells and tissue
  • NADH / FAD fluorescence dynamics
  • Viscosity imaging
  • Membrane dynamics
  • Membrane trafficking
  • LED inspection
  • Crude oil characterization


  • Lifetime accuracy as part of the LIFA system less than 30 ps
  • Fast switching between individual LEDs, no realignment of the lightsource or other manual interventions needed.
  • Digital modulation up to 80-120 MHz, depending on the wavelength
  • Choice between lens-coupling and fiber-coupling to your microscope
  • Integrated USB 2.0 control
  • Compatible with all major microscope brands
  • Dimensions (l x w x h): 346 x 109 x 183 mm

Temperature Stabilization

Increased temperature stabilization of the LED's is optionally available to minimize lifetime drifts during very long (many hours) time lapses.

Available wavelengths

All LEDs are high-quality modulating LEDs with a peak light intensity at wavelengths between 446 and 525 nm, 595 nm, 635 nm and 696 nm. Other wavelengths are available upon request.