The Lambert Instruments Multi-LED is a versatile pulsed excitation light source for fluorescence lifetime imaging microscopy in the frequency domain. The Multi-LED contains up to 4 LEDs that provide non-phototoxic illumination levels, have a low cost and a long economic lifespan.
Available wavelengths cover the range from 360 to 640 nm. The Multi-LED is part of the Lambert LIFA and LIFA-X products for fluorescence lifetime imaging microscopy/Forster Resonance Energy Transfer (FRET), and is seamlessly integrated with the LI-FLIM software. For each LED automatic control is available for LED selection, LED current and modulation frequency.
Oxygen concentration imaging in cells and tissue
NADH / FAD fluorescence dynamics
Crude oil characterization
Lifetime accuracy as part of the LIFA system less than 30 ps
Fast switching between individual LEDs, no realignment of the lightsource or other manual interventions needed.
Digital modulation up to 80-120 MHz, depending on the wavelength
Choice between lens-coupling and fiber-coupling to your microscope
Integrated USB 2.0 control
Compatible with all major microscope brands
Dimensions (l x w x h): 346 x 109 x 183 mm
Increased temperature stabilization of the LED's is optionally available to minimize lifetime drifts during very long (many hours) time lapses.
All LEDs are high-quality modulating LEDs with a peak light intensity at wavelengths between 446 and 525 nm, 595 nm, 635 nm and 696 nm. Other wavelengths are available upon request.