Bio-Formats 6.1 Release Introduces 12-Bit FLI-File Support

Bio-Formats 6.1 Release Introduces 12-Bit FLI-File Support


Bio-Formats 6.1.0 was released on 20 May 2019. This update introduces support for 12-bit FLI files recorded with the HiCAM Fluo and the Lambert HS1000.

When loading a 12-bit FLI file in ImageJ with Bio-Formats 6.1.0, the image data and the background will be loaded as separate stacks. The image data will be presented as a 16 bit stack in ImageJ, but the original 12 bit dynamic range of the image data will be preserved. This conversion supports virtual stacks to work on large files.

We recommend updating to the newest version of Bio-Formats to ensure optimal compatibility with the Lambert Instruments FLI file format.

For more information about what's new in Bio-Formats 6.1.0, please refer to the Bio-Formats version history.

Bio-Formats 6.0 Release Fixes FLI-File Import Issue

Bio-Formats 6.0 Release Fixes FLI-File Import Issue


Bio-Formats 6.0.0 was released on 18 February. This update includes a fix for a known issue where opening a large FLI-file could cause an integer overflow error. We recommend updating to the newest version of Bio-Formats to ensure optimal compatibility with the Lambert Instruments FLI file format.

For more information about what's new in Bio-Formats 6.0.0, please refer to the Bio-Formats version history.

Lambert Instruments Introduces sCMOS Camera for Effortless Scientific Imaging

Lambert sCMOS with lens.png

New camera combines excellent light sensitivity with automatic camera configuration

13 November 2018 - Groningen (NL) - Lambert Instruments today announced the introduction of a user-friendly sCMOS camera. The Lambert sCMOS automatically finds the optimal camera settings and captures excellent low-light images thanks to its advanced sCMOS image sensor.

Lambert sCMOS on microscope.png

With the Lambert sCMOS, Lambert Instruments aims to simplify image acquisition for scientific research, machine vision and industrial applications. "Researchers don't want to go through complicated camera configuration procedures before getting started with actually imaging their samples." says Jeroen Wehmeijer, Business Development Manager at Lambert Instruments. "That's why we paid a lot of attention to making the Lambert sCMOS a user-friendly scientific camera with great light sensitivity. The camera takes care of optimizing the images so the user can focus on their sample."

Automatic exposure and gain control adapt the camera configuration to match the current light conditions. And on-the-fly image correction and contrast enhancement ensure optimal image quality. In manual mode, users can fine-tune all camera settings for advanced applications that require quantitative image data.

The Lambert sCMOS features a 1280 x 1024 pixel sCMOS sensor that captures detailed images. Thanks to its excellent light sensitivity, the Lambert sCMOS captures up to 100 frames per second even under challenging low-light conditions.

With the Lambert Instruments Capture software, it is easy to record images and videos. Users can record, trim, analyze and export image data in widely supported image formats that are compatible with popular imaging platforms like Fiji/ImageJ.

For more information about the Lambert sCMOS, please visit or contact the Lambert Instruments sales team at or by calling +31 50 501 8461.

HiCATT now available with improved relay optics

The High-speed Intensified Camera Attachment (HiCATT) is an intensifier attachment that increases the light sensitivity of your high-speed camera. It is compatible with all popular high-speed cameras including the Photron and Phantom cameras.

The HiCATT is now available with improved relay optics. This new optical design further improves the light sensitivity of the HiCATT for excellent high-speed imaging performance in low light situations.

HiCATTs with 25 mm image intensifiers now include an improved optical design of the 1-to-1 (1:1) relay optics that offers light transmission improvements of up to 30%. In addition to the 1:1 relay optics, Lambert Instruments also offers 1.7:1 relay optics for optimal compatibility with smaller image sensors.

The HiCATT is compatible with all common high-speed camera mounts. Click the button below to see which HiCATT configuration if the best fit for your high-speed camera:


Lambert Instruments Introduces Toggel: A Next-Generation Fluorescence Lifetime Imaging Camera

New FLIM camera simplifies fluorescence lifetime imaging for researchers and imaging centers by combining excellent light sensitivity with easy image acquisition and data analysis.


5 April 2017 - Groningen (NL) - Lambert Instruments today announced the introduction of its next-generation fluorescence lifetime imaging camera: Toggel. This new camera makes fluorescence lifetime imaging microscopy (FLIM) and fluorescence resonance energy transfer (FRET) faster and easier with its excellent light sensitivity, sharp images and fast image acquisition.

With Toggel, Lambert Instruments aims to minimize measurement duration, automate image acquisition and simplify data analysis. These factors are of great importance to many of its customers in cell biology, cancer research and high-throughput screening.

"Toggel is the result of years of research and development." says Jasper Bosch, Development Engineer at Lambert Instruments. "From the start, we have collaborated with experts in FLIM and FRET at the Netherlands Cancer Institute, the University of Amsterdam and Delft University of Technology to make sure that this new camera fits the needs of our customers."

Toggel features a unique image sensor that was designed and optimized specifically for FLIM applications. This advanced sensor contains all the functionality required for fluorescence lifetime imaging, making the Toggel camera a solid-state FLIM camera and removing the need for an image intensifier. These technological improvements enable lifetime imaging at unprecedented frame rates with the single-image fluorescence lifetime imaging microscopy (siFLIM) method.*

"We used a prototype of this camera to develop a method for acquiring quantitative lifetime images from a single exposure." says professor Kees Jalink of the Netherlands Cancer Institute. "siFLIM takes advantage of the technical capabilities of this camera and simultaneously records two 180°-phase-shifted images. This allows for video-rate lifetime imaging with minimal phototoxicity and bleaching. siFLIM is also inherently immune to artifacts stemming from rapid cellular movements and signal transients."

Other imaging modes of the camera include regular frequency-domain FLIM acquisition and time-lapse recordings. Data analysis is done automatically by the Lambert Instruments software, which instantly calculates the fluorescence lifetime and presents it to the user as a colorized overlay on the original image.

For more information about Toggel, please visit or visit Lambert Instruments at booth 91 of the Focus On Microscopy conference in Bordeaux from 9-12 April.

* siFLIM: single-image frequency-domain FLIM provides fast and photon-efficient lifetime data, M. Raspe et al, Nature Methods 13, 501–504 (2016)

Toggel simplifies fluorescence lifetime imaging for researchers and imaging centers by combining excellent light sensitivity with easy image acquisition and data analysis.

Toggel simplifies fluorescence lifetime imaging for researchers and imaging centers by combining excellent light sensitivity with easy image acquisition and data analysis.

Lambert Instruments and FASTBASE SOLUTIONS Announce Strategic Partnership

FASTBASE SOLUTIONS implements a new cancer diagnostics platform based on proprietary technology delivered through the Lambert Instruments FLIM Attachment (LIFA) technology.

30 March 2017, Groningen (NL) - FASTBASE SOLUTIONS has developed a high specificity, super-sensitive, quantitative biomarker platform employing amplified FRET. This platform is designed to report on protein activation states and protein-complex formation in situ, such as in tumor biopsies. The mission of FASTBASE SOLUTIONS is to be the leading platform in cancer diagnostics, bringing precision to cancer patient treatment.

The image acquisition and analysis platform that FASTBASE SOLUTIONS employs is based on the fluorescence lifetime imaging technology developed by Lambert Instruments. Both parties have agreed to enter into a strategic partnership in order to successfully market FASTBASE SOLUTIONS technologies.

Professor Banafshé Larijani, CSO of FASTBASE: “The Lambert Instruments FLIM technology provides us with the tools we need to build our quantitative biomarker platform and accelerate our growth as a young company.”

Business Development Manager of Lambert Instruments, Jeroen Wehmeijer: “We are pleased to support Fastbase Solutions and their innovative applications of our FLIM technology.”

Find out more about the FASTBASE application at


About Lambert Instruments ( )
Lambert Instruments B.V., founded in 1992, located in Groningen, the Netherlands, develops advanced imaging solutions for low-light applications. Its flagship product is the Lambert Instruments FLIM Attachment (LIFA), which is a turn-key solution for fluorescence lifetime imaging microscopy (FLIM).  With end-users at all major universities and research institutes worldwide, Lambert Instruments has a solid reputation for high-end imaging solutions.

FASTBASE SOLUTIONS, is located in Bic Bizkaia 612, 48160 Derio, in the Basque Country, Spain. It was founded in 2016, by Peter J Parker, Banafshé Larijani, Pierre Leboucher and Antonio Ferrer. FASTBASE SOLUTIONS is implementing new technology to provide functional proteomics insights. This proprietary technology is delivered through the Lambert Instruments FLIM Attachment (LIFA) technology.  

Contact Information

Both Lambert Instruments and FASTBASE Solutions will be present during the Focus On Microscopy conference in Bordeaux at booth 91. For further information please contact Lambert Instruments at +31 50 501 8461.


New Image Intensifiers Available

We are excited to announce that four new image intensifiers are now available for all our intensified imaging solutions. These second generation image intensifiers have a higher quantum efficiency than the regular second generation image intensifiers.

Spectral Sensitivity

Each of the new image intensifiers covers a different part of the spectrum:

  • Hi-QE Red has a spectral sensitivity that covers most of the visible part of the spectrum. Its spectral sensitivity covers a range of wavelengths that is similar to an S25 or GaAs image intensifier. In terms of quantum efficiency, the Hi-QE Red image intensifier is positioned between the S25 and GaAs intensifiers.
  • Hi-QE Green has a peak quantum efficiency of more than 30%. Its spectral sensitivity covers the lower half of the visible spectrum.
  • Hi-QE Blue is sensitive to blue light and UV light down to wavelengths of approximately 200 nm.
  • Hi-QE UV is similar to the Hi-QE Blue image intensifier, but its sensitivity also covers shorter wavelengths in the UV part of the spectrum.

Gating Specifications

These new image intensifiers have a minimum gate width of 500 ns and are only available with a diameter of 18 mm.

More information

For more information about these new image intensifiers, please refer to this page about image intensifier photocathodes.

New Bio-Formats Release Fixes FLI-File Import Issue

New Bio-Formats Release Fixes FLI-File Import Issue

Bio-Formats 5.2.0 was released at the end of August. This update includes a fix for a known issue where opening a FLI-file could cause an error message. We recommend updating to the newest version of Bio-Formats to ensure optimal compatibility with the Lambert Instruments FLI file format.

For more information about what's new in Bio-Formats 5.2.0, please refer to the Bio-Formats version history.

Kick-Off Health Hub Roden

Kick-Off Health Hub Roden

Op 13 maart 2015 werd het ambitieuze project Health Hub Roden feestelijk gelanceerd. Nu, bijna een jaar later, is er veel veranderd. De verbouwing van de locatie in het voormalig pand van Cordis is gereed. Het aantal aangesloten bedrijven overtreft de prognoses ruimschoots. Tijd om dit eerste jaar feestelijk af te sluiten.

High Speed, High Standards

As sensor resolutions and frame rates increase, so does the amount of data that is recorded by digital cameras. High-speed cameras like the HiCAM Fluo record hundreds of frames per second at high resolution. This results in nearly 1.5 GB of data per second. The camera streams all this data directly to your computer over the CoaXPress interface. This is a camera industry standard that can transfer several GB/s. But there are more industry standards for imaging devices. Lambert Instruments implements these standards to ensure that our products are easy to implement.

Click any of the logo's below to find out more about these standards.


Our sales team is available to answer any questions you may have about camera interfaces. We also have a calculator that helps you find out how much data your camera is recording so you can pick the right interface.

Intensified Imaging for Combustion Research

Researchers around the world are using the HiCATT in their combustion studies. The light intensity of flames is not very high, and very short exposure times are needed to see any details. The images below show three recordings of a blue gas flame: image A is a regular recording of a blue gas flame. It shows the general shape of the flame, but the details are lost due to the long exposure time.

Image B was recorded with a high-speed camera at 1000 fps with 1 millisecond exposure time. This image shows some of the fine details of the flame shape, but it is a little dim and blurry. The high-speed camera does a decent job at preventing motion blur by having a short exposure time. But because of this short exposure time, the camera can't collect enough light to capture a detailed image.

Image C shows what a flame looks like when using a 15 microsecond exposure time. To achieve such a short exposure time but retain image detail, we used the fast electro-optical shutter function of the image intensifier. This technique is called gating, and it allows for ultra-short exposure times. During this short exposure time, the image intensifier boosts the intensity of the incoming light to retain image detail.

This unique imaging technology enables experimental investigation of combustion and flame structure. For more information about applications of image intensifier gating for combustion research please have a look at these HiCATT user publications:


Baptiste Déjean, Pierre Berthoumieu and Pierre Gajan, Experimental study on the influence of liquid and air boundary conditions on a planar air-blasted liquid sheet, Part II: prefilming zone length in the International Journal of Multiphase Flow


Shigeru Tachibana, Kinya Saito, Takeshi Yamamoto, Mitsumasa Makida, Tomoaki Kitano and Ryoichi Kurose, Experimental and numerical investigation of thermo-acoustic instability in a liquid-fuel aero-engine combustor at elevated pressure: Validity of large-eddy simulation of spray combustion in Combustion and Flame


Min Jung Lee, Moon Soo Cho and Nam Il Kim, Characteristics of opposed flow partially premixed flames in mesoscale channels at low strain rates in Proceedings of the Combustion Institute


For a full overview of HiCATT user publications, please visit our HiCATT User Publications page.

Introducing the New TRiCAM

Lambert Instruments is proud to announce the next generation of its time-resolved intensified camera. The new TRiCAM is designed for scientific and industrial applications that require low-light imaging. It features a fast sensor, a high-performance image intensifier, and a redesigned aluminum enclosure.

Fast CMOS Sensor

The sensor in the new TRiCAM captures up to 160 frames per second at full resolution, and it can reach over 1000 fps at reduced resolution. Thanks to its electronic global shutter readout method, the sensor reads out all 2.3 million pixels at the same time. This prevents rolling shutter effects and ensures optimal image quality.

High-Performance Image Intensifier

Fiber-optically attached to the sensor is a high-performance image intensifier. This incredibly perceptive intensifier boosts the incoming light, allowing you to capture detailed images in the most challenging light conditions.

The built-in electronics offer a wide range of functionalities. Ultra-fast gating enables exposure times down to 3 nanoseconds. By precisely timing these exposures, the TRiCAM can be used for applications like time-gated luminescence and laser-induced fluorescence. With modulation frequencies up to 120 MHz, the TRiCAM is also ideal for a wide range of frequency-domain applications.

Redesigned Aluminum Enclosure

The aluminum enclosure of the TRiCAM has been redesigned from scratch. While remaining as compact as before, the TRiCAM can now be configured with an 18 mm intensifier or a 25 mm intensifier. It is compatible with both C-mount and F-mount input, and the standard tripod thread.

Machine Vision Standards

The TRiCAM is available with a USB3 or Ethernet interface. To provide optimal integration with your existing setup, it supports USB3 Vision and GigE machine vision standards.


The new TRiCAM is available as of today. Our experienced sales engineers are happy to assist you with choosing the right image intensifier for your application. Please contact our sales team or one of our distributors for more information.

How to Gain New Insights with Super-Slow Motion Imaging

How to Gain New Insights with Super-Slow Motion Imaging

When things move too fast, there's nothing better than a super-slow motion replay to see what's going on. The ongoing development of high-speed image sensors gives us the ability to slow down fast-moving objects and highly dynamic processes. By taking a closer look at the slow-motion footage, we can gain unprecedented insight into these phenomena.

For industrial applications like machine welding, super-slow motion video can be used for quality control or to find the cause of defects. The video below offers a detailed look at the machine-welding process.

The Lambert HS540 Series provide a versatile solution for super-slow motion imaging. With its 2.9 megapixel sensor, you record detailed videos at thousands of frames per second.

For low-light applications, the HiCAM series offers an intensified solution for super-slow motion recordings when little light is available.

Lambert Instruments Introduces New High-Speed Cameras

Lambert Instruments is proud to announce the introduction of a new line of high-speed cameras: The Lambert HS Series. The first cameras in the Lambert HS series feature a fast high-resolution sensor. The Lambert HS540M and the Lambert HS540S offer simple and efficient high-speed imaging for scientific research, R&D, machine vision and other industrial applications.

Lambert HS540M
The Lambert HS540M is a high-speed camera for research applications. It has up to 16 GB of internal storage and is ideal for scientific research and industrial R&D. After recording your data, you can review the results in our software and trim the high-speed video before exporting it to your computer.

Lambert HS540S
The Lambert HS540S is a streaming high-speed camera for industrial applications. It is designed for high-performance tasks like machine vision, quality control and wafer inspection. Instead of saving the images to internal storage, the camera streams high-speed video directly to your computer over a CoaXPress (CXP) interface. And with Power over CXP (PoCXP) the camera can be powered over the CoaXPress channels, removing the need for a dedicated power cable.

Fast High-Resolution Sensor
Both the Lambert HS540M and the HS540S feature a high-speed CMOS sensor that records full-resolution images (1696 x 1710 pixels) at 540 fps. To increase the framerate, the camera can use a smaller part of the sensor to reduce the image resolution. By doing so, it can deliver framerates of up to 170000 frames per second. Its electronic global shutter and minimum exposure time of 2 us ensure sharp images of fast-moving objects.

Digital Imaging Standards
The Lambert HS540 cameras support digital imaging standards that make it easy to integrate into an automated setup. The Generic Interface for Cameras (GenICam) offers a universal programming interface for controlling the camera settings and retrieving the recorded data. Camera characteristics are determined using the measurement procedures from the EMVA 1288 Standard. This makes specifications more transparent and easier to compare to other cameras.

New Business Location

New Business Location

Lambert Instruments is moving to a new location on May 1. Our new office building in Groningen is easier to reach for customers and it is more suited to accommodate our ongoing efforts to develop advanced imaging solutions.

Our new address is:

Lambert Instruments BV
Leonard Springerlaan 19
9727KB Groningen
The Netherlands

Our phone number will remain unchanged: +31 50 501 8461.

During this transition we might not respond to your emails as quickly as you're used to from us. We apologize for any inconvenience this might cause and will try to respond as soon as we can.

Introducing a Time-Domain FLIM Solution for Widefield Microscopes

Lambert Instruments is proud to introduce a new fluorescence lifetime imaging microscopy system: The Lambert Instruments FLIM Attachment for Time-Domain imaging. The LIFA-TD offers a turn-key solution for fluorescence lifetime imaging microscopy and is compatible with every widefield fluorescence microscope. From recording the data to calculating the fluorescence lifetime, the entire measurement procedure is automated by our advanced software.

The LIFA-TD includes a camera with a built-in image intensifier, which can precisely control the exposure of the camera. With its advanced gating capabilities, the image intensifier can reduce the exposure time to 3 ns and alter the timing of the exposure in the subnanosecond range.

A pulsed laser excites the fluorophores in the sample. By using a laser with a small pulse width, the accuracy of the lifetime measurements can be optimized.

Images are recorded with our advanced software, which also processes the data to determine the fluorescence lifetime. The fluorescence lifetimes are then shown as a colorized overlay on the original image.

No More Motion Blur

High-speed cameras are great for recording highly dynamic events. Experts in ballistics research, combustion analysis and in-vivo imaging require fast cameras for their work. The high frame rates give them unprecedented insight into what happens in the blink of an eye.

But even the best high-speed cameras have their limits. In some situations even the fastest cameras are not fast enough. Objects move too fast or emit too little light to produce a crisp image. The result is a dim or blurry picture that hardly contains any useful information.

These situations call for a high-tech solution that offers extremely short exposure times or extreme light sensitivity. With an image intensifier you can use its gating feature to achieve exposure times down to 3 ns, completely eliminating motion blur. With such a short exposure time, even very fast objects can be imaged clearly. And because the image intensifier amplifies the recorded light, you don't have to worry about underexposure.

The Lambert Instruments HiCATT upgrades your high-speed camera to the next level of performance. It is compatible with any high-speed camera and all popular lens mounts. Its image intensifier component is carefully selected to match your desired frame rate and light sensitivity. Our custom software lets you control the exposure time and the gain of the HiCATT to find the right settings for your application.

Engineers and researchers around the world use the HiCATT to study bullet trajectories, combustion processes in fuel injection engines, and voltage sensitive dyes in living tissue. Find out more about HiCATT applications and technical information.

How To Freeze A Flame: Watching Combustion in Super-Slow Motion

Some things happen too fast for the human eye to see. The blades of a spinning fan, for example, look like a blur to us. They move too quickly for our eyes to see them the way we do when the fan is off.

The same thing happens when we look at a flame. It may look like a slowly waving sheet of warm light to the human eye, but in reality it is a swirling chemical process that changes faster than we can see. Even digital cameras have a hard time recording combustion processes.

The dynamic nature of the flames requires a high-speed camera that can record thousands of images per second with each one of those images recorded in a fraction of a second. But while images can be captured during those brief periods, a regular high-speed camera can’t capture enough light to produce a clear image. It’s a challenge that combustion researchers have struggled with for a long time.

Combustion cycle of a fuel injection engine.

Combustion cycle of a fuel injection engine.

The solution is a camera that is both fast and extremely sensitive to light. We combined these advanced features into one system with the HiCAM, a high-speed camera with an unparalleled light sensitivity. It records clear images in an instant and produces super-slow motion videos that accurately show the behavior of a flame. With the HiCAM, researchers are now able to quantitatively study combustion processes in combustion burners and in fuel injection engines.

Gain Control for Automation Systems

Introducing a new control unit for intensifier attachments: The gain control unit for automated systems enables full control over the gain and anode current limit of the image intensifier. Communication over TTL and analog signals provides precise timing and ensures straightforward integration of an intensifier into your imaging system. And with its built-in protection features, the control unit helps to protect the image intensifier from overexposure.

The gain control unit for automation systems is now available with our HiCATT and TRiCATT intensified camera attachments.