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LIFA

Bio-Formats 6.1 Release Introduces 12-Bit FLI-File Support

Bio-Formats 6.1 Release Introduces 12-Bit FLI-File Support

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Bio-Formats 6.1.0 was released on 20 May 2019. This update introduces support for 12-bit FLI files recorded with the HiCAM Fluo and the Lambert HS1000.

When loading a 12-bit FLI file in ImageJ with Bio-Formats 6.1.0, the image data and the background will be loaded as separate stacks. The image data will be presented as a 16 bit stack in ImageJ, but the original 12 bit dynamic range of the image data will be preserved. This conversion supports virtual stacks to work on large files.

We recommend updating to the newest version of Bio-Formats to ensure optimal compatibility with the Lambert Instruments FLI file format.

For more information about what's new in Bio-Formats 6.1.0, please refer to the Bio-Formats version history.

Bio-Formats 6.0 Release Fixes FLI-File Import Issue

Bio-Formats 6.0 Release Fixes FLI-File Import Issue

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Bio-Formats 6.0.0 was released on 18 February. This update includes a fix for a known issue where opening a large FLI-file could cause an integer overflow error. We recommend updating to the newest version of Bio-Formats to ensure optimal compatibility with the Lambert Instruments FLI file format.

For more information about what's new in Bio-Formats 6.0.0, please refer to the Bio-Formats version history.

Lambert Instruments Introduces Toggel: A Next-Generation Fluorescence Lifetime Imaging Camera

New FLIM camera simplifies fluorescence lifetime imaging for researchers and imaging centers by combining excellent light sensitivity with easy image acquisition and data analysis.

 

5 April 2017 - Groningen (NL) - Lambert Instruments today announced the introduction of its next-generation fluorescence lifetime imaging camera: Toggel. This new camera makes fluorescence lifetime imaging microscopy (FLIM) and fluorescence resonance energy transfer (FRET) faster and easier with its excellent light sensitivity, sharp images and fast image acquisition.

With Toggel, Lambert Instruments aims to minimize measurement duration, automate image acquisition and simplify data analysis. These factors are of great importance to many of its customers in cell biology, cancer research and high-throughput screening.

"Toggel is the result of years of research and development." says Jasper Bosch, Development Engineer at Lambert Instruments. "From the start, we have collaborated with experts in FLIM and FRET at the Netherlands Cancer Institute, the University of Amsterdam and Delft University of Technology to make sure that this new camera fits the needs of our customers."

Toggel features a unique image sensor that was designed and optimized specifically for FLIM applications. This advanced sensor contains all the functionality required for fluorescence lifetime imaging, making the Toggel camera a solid-state FLIM camera and removing the need for an image intensifier. These technological improvements enable lifetime imaging at unprecedented frame rates with the single-image fluorescence lifetime imaging microscopy (siFLIM) method.*

"We used a prototype of this camera to develop a method for acquiring quantitative lifetime images from a single exposure." says professor Kees Jalink of the Netherlands Cancer Institute. "siFLIM takes advantage of the technical capabilities of this camera and simultaneously records two 180°-phase-shifted images. This allows for video-rate lifetime imaging with minimal phototoxicity and bleaching. siFLIM is also inherently immune to artifacts stemming from rapid cellular movements and signal transients."

Other imaging modes of the camera include regular frequency-domain FLIM acquisition and time-lapse recordings. Data analysis is done automatically by the Lambert Instruments software, which instantly calculates the fluorescence lifetime and presents it to the user as a colorized overlay on the original image.

For more information about Toggel, please visit www.lambertinstruments.com/toggel or visit Lambert Instruments at booth 91 of the Focus On Microscopy conference in Bordeaux from 9-12 April.

* siFLIM: single-image frequency-domain FLIM provides fast and photon-efficient lifetime data, M. Raspe et al, Nature Methods 13, 501–504 (2016)

Toggel simplifies fluorescence lifetime imaging for researchers and imaging centers by combining excellent light sensitivity with easy image acquisition and data analysis.

Toggel simplifies fluorescence lifetime imaging for researchers and imaging centers by combining excellent light sensitivity with easy image acquisition and data analysis.

Introducing a Time-Domain FLIM Solution for Widefield Microscopes

Lambert Instruments is proud to introduce a new fluorescence lifetime imaging microscopy system: The Lambert Instruments FLIM Attachment for Time-Domain imaging. The LIFA-TD offers a turn-key solution for fluorescence lifetime imaging microscopy and is compatible with every widefield fluorescence microscope. From recording the data to calculating the fluorescence lifetime, the entire measurement procedure is automated by our advanced software.

The LIFA-TD includes a camera with a built-in image intensifier, which can precisely control the exposure of the camera. With its advanced gating capabilities, the image intensifier can reduce the exposure time to 3 ns and alter the timing of the exposure in the subnanosecond range.

A pulsed laser excites the fluorophores in the sample. By using a laser with a small pulse width, the accuracy of the lifetime measurements can be optimized.

Images are recorded with our advanced software, which also processes the data to determine the fluorescence lifetime. The fluorescence lifetimes are then shown as a colorized overlay on the original image.

Lambert Instruments Celebrates the 10th Anniversary of the LIFA

This year marks the 10th anniversary of the LIFA. With the first Lambert Instruments FLIM Attachment (LIFA) a decade ago, we introduced an easy and fast approach to fluorescence lifetime imaging. Since then, we advanced our imaging and analysis software; we improved our hardware and made it more compact; and we added compatibility with third-party hardware. But at the heart of the LIFA experience are still the features that matter most to our users. They are using the LIFA every day, because it is the easiest and fastest system for fluorescence lifetime imaging microscopy.

Read our interview with Dr. Kees Jalink at the Netherlands Cancer Institute about his experiences with the LIFA.

Lambert Instruments Ships First LIFA to India

Lambert Instruments is proud to announce the shipment of the first LIFA to India, following our increased effort to meet the growing demand for scientific imaging equipment in Asia.

Professor Inamdar studies quantum-dot-based FRET at Karnatak University in Dharwad, India. His interest in the LIFA was sparked by its ability to quickly measure fluorescence lifetimes. "We used to do FRET measurements using fluorescence intensities," says professor Inamdar, "but with ratiometric techniques you can't measure fluorescence lifetimes. So we started looking for a FLIM system, because fluorescence lifetimes are very useful for our kind of work."

With the LIFA, Inamdar hopes to advance his research and extend it to biological applications. The LIFA is the ideal solution for such applications, because it can record a lifetime image in a matter of seconds, practically making the process a real-time experience.

Inamdar was supported by Dynotech Instruments during the purchase process. Dynotech Instruments is a leading supplier of products and solutions in the field of optics in India, including Lambert Instruments products.

From 23rd to 25th of September, Lambert Instruments and Dynotech Instruments will be at the Laser World of Photonics 2014 in Bangalore. Visit us at booth B006 in hall 3A for more information about the LIFA and our other products.

FLIM-FRET Workshop in Glasgow

Lambert Instruments invites you to register for the FLIM-FRET workshop on September 3 and 4 at the Beatson Institute for Cancer Research in Glasgow. The event is hosted by the Beatson Advanced Imaging Resource and will be suitable for all levels of experience.
 

Program

September 3 will consist of a full program with lectures and hands-on sessions from experts in FLIM and FRET. The second day of the workshop is optional and reserved for attendees who would like a more in-depth fluorescence microscopy training. You are welcome to bring your own samples to the sessions on the second day.
 

Further information

For further information regarding registration and hotels, please refer to the workshop flyer.

Application note: Oxygen transport using phosphorescence lifetime imaging

We have published a new application note on the use of phosphorescence lifetime imaging of oxygen transport across slippery and curved gas-liquid interfaces. The note describes the experiments performed by Dr. Elif Karatay during her PhD studies at the University of Twente (NL). She used an oxygen sensitive luminescent dye to image the oxygen concentration in flowing aqueous solutions. The eXtended Lambert Instruments FLIM Attachment (LIFA-X) enabled her to image lifetimes up to 1 ms.

Read more: Oxygen transport using phosphorescence lifetime imaging.

Dr. Karatay is currently doing her postdoctoral research at Stanford University in the United States.