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Bio-Formats 6.1 Release Introduces 12-Bit FLI-File Support

Bio-Formats 6.1 Release Introduces 12-Bit FLI-File Support


Bio-Formats 6.1.0 was released on 20 May 2019. This update introduces support for 12-bit FLI files recorded with the HiCAM Fluo and the Lambert HS1000.

When loading a 12-bit FLI file in ImageJ with Bio-Formats 6.1.0, the image data and the background will be loaded as separate stacks. The image data will be presented as a 16 bit stack in ImageJ, but the original 12 bit dynamic range of the image data will be preserved. This conversion supports virtual stacks to work on large files.

We recommend updating to the newest version of Bio-Formats to ensure optimal compatibility with the Lambert Instruments FLI file format.

For more information about what's new in Bio-Formats 6.1.0, please refer to the Bio-Formats version history.

Bio-Formats 6.0 Release Fixes FLI-File Import Issue

Bio-Formats 6.0 Release Fixes FLI-File Import Issue


Bio-Formats 6.0.0 was released on 18 February. This update includes a fix for a known issue where opening a large FLI-file could cause an integer overflow error. We recommend updating to the newest version of Bio-Formats to ensure optimal compatibility with the Lambert Instruments FLI file format.

For more information about what's new in Bio-Formats 6.0.0, please refer to the Bio-Formats version history.

New Image Intensifiers Available

We are excited to announce that four new image intensifiers are now available for all our intensified imaging solutions. These second generation image intensifiers have a higher quantum efficiency than the regular second generation image intensifiers.

Spectral Sensitivity

Each of the new image intensifiers covers a different part of the spectrum:

  • Hi-QE Red has a spectral sensitivity that covers most of the visible part of the spectrum. Its spectral sensitivity covers a range of wavelengths that is similar to an S25 or GaAs image intensifier. In terms of quantum efficiency, the Hi-QE Red image intensifier is positioned between the S25 and GaAs intensifiers.
  • Hi-QE Green has a peak quantum efficiency of more than 30%. Its spectral sensitivity covers the lower half of the visible spectrum.
  • Hi-QE Blue is sensitive to blue light and UV light down to wavelengths of approximately 200 nm.
  • Hi-QE UV is similar to the Hi-QE Blue image intensifier, but its sensitivity also covers shorter wavelengths in the UV part of the spectrum.

Gating Specifications

These new image intensifiers have a minimum gate width of 500 ns and are only available with a diameter of 18 mm.

More information

For more information about these new image intensifiers, please refer to this page about image intensifier photocathodes.

New Bio-Formats Release Fixes FLI-File Import Issue

New Bio-Formats Release Fixes FLI-File Import Issue

Bio-Formats 5.2.0 was released at the end of August. This update includes a fix for a known issue where opening a FLI-file could cause an error message. We recommend updating to the newest version of Bio-Formats to ensure optimal compatibility with the Lambert Instruments FLI file format.

For more information about what's new in Bio-Formats 5.2.0, please refer to the Bio-Formats version history.

Kick-Off Health Hub Roden

Kick-Off Health Hub Roden

Op 13 maart 2015 werd het ambitieuze project Health Hub Roden feestelijk gelanceerd. Nu, bijna een jaar later, is er veel veranderd. De verbouwing van de locatie in het voormalig pand van Cordis is gereed. Het aantal aangesloten bedrijven overtreft de prognoses ruimschoots. Tijd om dit eerste jaar feestelijk af te sluiten.

High Speed, High Standards

As sensor resolutions and frame rates increase, so does the amount of data that is recorded by digital cameras. High-speed cameras like the HiCAM Fluo record hundreds of frames per second at high resolution. This results in nearly 1.5 GB of data per second. The camera streams all this data directly to your computer over the CoaXPress interface. This is a camera industry standard that can transfer several GB/s. But there are more industry standards for imaging devices. Lambert Instruments implements these standards to ensure that our products are easy to implement.

Click any of the logo's below to find out more about these standards.


Our sales team is available to answer any questions you may have about camera interfaces. We also have a calculator that helps you find out how much data your camera is recording so you can pick the right interface.

Intensified Imaging for Combustion Research

Researchers around the world are using the HiCATT in their combustion studies. The light intensity of flames is not very high, and very short exposure times are needed to see any details. The images below show three recordings of a blue gas flame: image A is a regular recording of a blue gas flame. It shows the general shape of the flame, but the details are lost due to the long exposure time.

Image B was recorded with a high-speed camera at 1000 fps with 1 millisecond exposure time. This image shows some of the fine details of the flame shape, but it is a little dim and blurry. The high-speed camera does a decent job at preventing motion blur by having a short exposure time. But because of this short exposure time, the camera can't collect enough light to capture a detailed image.

Image C shows what a flame looks like when using a 15 microsecond exposure time. To achieve such a short exposure time but retain image detail, we used the fast electro-optical shutter function of the image intensifier. This technique is called gating, and it allows for ultra-short exposure times. During this short exposure time, the image intensifier boosts the intensity of the incoming light to retain image detail.

This unique imaging technology enables experimental investigation of combustion and flame structure. For more information about applications of image intensifier gating for combustion research please have a look at these HiCATT user publications:


Baptiste Déjean, Pierre Berthoumieu and Pierre Gajan, Experimental study on the influence of liquid and air boundary conditions on a planar air-blasted liquid sheet, Part II: prefilming zone length in the International Journal of Multiphase Flow


Shigeru Tachibana, Kinya Saito, Takeshi Yamamoto, Mitsumasa Makida, Tomoaki Kitano and Ryoichi Kurose, Experimental and numerical investigation of thermo-acoustic instability in a liquid-fuel aero-engine combustor at elevated pressure: Validity of large-eddy simulation of spray combustion in Combustion and Flame


Min Jung Lee, Moon Soo Cho and Nam Il Kim, Characteristics of opposed flow partially premixed flames in mesoscale channels at low strain rates in Proceedings of the Combustion Institute


For a full overview of HiCATT user publications, please visit our HiCATT User Publications page.

How to Gain New Insights with Super-Slow Motion Imaging

How to Gain New Insights with Super-Slow Motion Imaging

When things move too fast, there's nothing better than a super-slow motion replay to see what's going on. The ongoing development of high-speed image sensors gives us the ability to slow down fast-moving objects and highly dynamic processes. By taking a closer look at the slow-motion footage, we can gain unprecedented insight into these phenomena.

For industrial applications like machine welding, super-slow motion video can be used for quality control or to find the cause of defects. The video below offers a detailed look at the machine-welding process.

The Lambert HS540 Series provide a versatile solution for super-slow motion imaging. With its 2.9 megapixel sensor, you record detailed videos at thousands of frames per second.

For low-light applications, the HiCAM series offers an intensified solution for super-slow motion recordings when little light is available.

No More Motion Blur

High-speed cameras are great for recording highly dynamic events. Experts in ballistics research, combustion analysis and in-vivo imaging require fast cameras for their work. The high frame rates give them unprecedented insight into what happens in the blink of an eye.

But even the best high-speed cameras have their limits. In some situations even the fastest cameras are not fast enough. Objects move too fast or emit too little light to produce a crisp image. The result is a dim or blurry picture that hardly contains any useful information.

These situations call for a high-tech solution that offers extremely short exposure times or extreme light sensitivity. With an image intensifier you can use its gating feature to achieve exposure times down to 3 ns, completely eliminating motion blur. With such a short exposure time, even very fast objects can be imaged clearly. And because the image intensifier amplifies the recorded light, you don't have to worry about underexposure.

The Lambert Instruments HiCATT upgrades your high-speed camera to the next level of performance. It is compatible with any high-speed camera and all popular lens mounts. Its image intensifier component is carefully selected to match your desired frame rate and light sensitivity. Our custom software lets you control the exposure time and the gain of the HiCATT to find the right settings for your application.

Engineers and researchers around the world use the HiCATT to study bullet trajectories, combustion processes in fuel injection engines, and voltage sensitive dyes in living tissue. Find out more about HiCATT applications and technical information.

How To Freeze A Flame: Watching Combustion in Super-Slow Motion

Some things happen too fast for the human eye to see. The blades of a spinning fan, for example, look like a blur to us. They move too quickly for our eyes to see them the way we do when the fan is off.

The same thing happens when we look at a flame. It may look like a slowly waving sheet of warm light to the human eye, but in reality it is a swirling chemical process that changes faster than we can see. Even digital cameras have a hard time recording combustion processes.

The dynamic nature of the flames requires a high-speed camera that can record thousands of images per second with each one of those images recorded in a fraction of a second. But while images can be captured during those brief periods, a regular high-speed camera can’t capture enough light to produce a clear image. It’s a challenge that combustion researchers have struggled with for a long time.

Combustion cycle of a fuel injection engine.

Combustion cycle of a fuel injection engine.

The solution is a camera that is both fast and extremely sensitive to light. We combined these advanced features into one system with the HiCAM, a high-speed camera with an unparalleled light sensitivity. It records clear images in an instant and produces super-slow motion videos that accurately show the behavior of a flame. With the HiCAM, researchers are now able to quantitatively study combustion processes in combustion burners and in fuel injection engines.

Updated Lambert Instruments Website

We have updated the Lambert Instruments website. It has been redesigned and optimized for both desktop computers and mobile devices. 

The product pages have been updated to give a simple overview of the features and specifications of our products. For further information, there are downloadable specifications available for most products. And if you have other questions about a product, you can simply press the green button at the top of each product page to contact us.

All the application notes and technology notes have also been moved to the updated website. We categorized and tagged them, so it's easier to find the information you are looking for. After clicking Applications or Technologies in the navigation bar, you will see an overview of the most recent posts. When you scroll down, you can select a category that describes the field or the product you are interested in. If you want to learn about a specific subject, you can click one of the tags. And if the categories and tags don't list the subject you are looking for, then you can always use the search field:


If you have any questions about the updated website, then please let us know.

Lambert Instruments Celebrates the 10th Anniversary of the LIFA

This year marks the 10th anniversary of the LIFA. With the first Lambert Instruments FLIM Attachment (LIFA) a decade ago, we introduced an easy and fast approach to fluorescence lifetime imaging. Since then, we advanced our imaging and analysis software; we improved our hardware and made it more compact; and we added compatibility with third-party hardware. But at the heart of the LIFA experience are still the features that matter most to our users. They are using the LIFA every day, because it is the easiest and fastest system for fluorescence lifetime imaging microscopy.

Read our interview with Dr. Kees Jalink at the Netherlands Cancer Institute about his experiences with the LIFA.

Newsletter: How to Fingerprint Oils and Tell Them Apart

Quality control is an important part of any production process. A series of checks on a randomly selected product tells you whether its quality meets the required standards. The TRiCAM is a fast and accurate instrument for quality control.

Taotao Mu at the Beijing Institute of Technology developed a method to quickly determine the fluorescent fingerprint of both motor oils and edible oils. Consistent production quality is critical for these products, making quick and reliable quality control all the more important.

Mu uses a gated TRiCAM and a pulsed laser for his experiments. He studies the decay of the fluorescence light intensity after excitation at several wavelengths. From this data, he compiles a contour diagram of fluorescence intensity: the equivalent of a fluorescent fingerprint of the oil. This allows him to tell apart wide varieties of oils.

Contour diagrams of fluorescence intensities of (a) olive, (b) rapeseed, (c) grapeseed, (d) soybean, (e) corn, (f, g, h) peanut oils. The fluorescence wavelength and detection time after the excitation light pulse are used as axes.

The TRiCAM can record images using gate widths down to less than 3 ns. By carefully synchronizing the laser and the camera, the decay of the fluorescence light intensity can be studied in great detail.

For more information, please refer to our application note about time-resolved fluorescence in the analysis of edible oils.

Newsletter: Intensify Your Camera

Does your camera struggle to record clear images in low-light situations? You could increase the exposure time to capture more light. But that may introduce motion blur. What you need is a camera with a better light sensitivity. Instead of buying a new camera, you can improve the light sensitivity of your current camera by equipping it with an image intensifier. Upgrading your camera in this way saves you the trouble of implementing a new camera into your setup.

The image below illustrates the effect of the image intensifier. It shows images of a coffee mug that we recorded with and without an image intensifier in a room with little light. The left part of the image was recorded with a camera without an image intensifier. The right part was recorded with the same camera, but with an intensifier coupled to the sensor. The gain of the intensifier was set to a low value, so the light intensity can still be boosted much further.

Note how much brighter the right part of the image is, despite the fact that it was recorded with a shorter exposure time than the left part of the image.

Our new Intensify Your Camera service is the easiest way to boost the light sensitivity of your camera. Based on the specifications of your camera and the optical requirements for your setup, we will make a detailed recommendation for the required image intensifier and optics. You then send your camera to Lambert Instruments. Our skilled technicians will fiber-optically couple the image intensifier to your camera. They have years of experience doing this because we use the same technique for our own camera systems.

The image intensifier increases your camera's light sensitivity by orders of magnitude. And it enables nanosecond exposures when combined with our advanced gating technologies. With such short exposure times motion blur is no longer an issue, not even in low-light situations. And because we attach the image intensifier to your own camera, you can keep using the software and analysis methods you are already familiar with.

Lambert Instruments has over two decades of experience with image intensifiers. We use them in our imaging systems and have equipped many types of sensors with image intensifiers. You can read about coupling an image intensifier to a sensor or if you are interested in adding an image intensifier to your camera, you can submit your camera specifications.

Application note: Time-resolved fluorescence in the analysis of edible oils

Taotao Mu at the Beijing Institute of Technology used a TRiCAM to make contour diagrams of the time-resolved fluorescence intensities of different kinds of edible oils. This method greatly improves the identification capabilities and outperforms traditional fluorescence analysis.

In the application note 'Time-resolved fluorescence in the analysis of edible oils' you can read all about the advantages of the short gating periods the TRiCAM enables.

Application note: Oxygen transport using phosphorescence lifetime imaging

We have published a new application note on the use of phosphorescence lifetime imaging of oxygen transport across slippery and curved gas-liquid interfaces. The note describes the experiments performed by Dr. Elif Karatay during her PhD studies at the University of Twente (NL). She used an oxygen sensitive luminescent dye to image the oxygen concentration in flowing aqueous solutions. The eXtended Lambert Instruments FLIM Attachment (LIFA-X) enabled her to image lifetimes up to 1 ms.

Read more: Oxygen transport using phosphorescence lifetime imaging.

Dr. Karatay is currently doing her postdoctoral research at Stanford University in the United States.

Tutorial: Automated FLIM

Lambert Instruments keeps improving and expanding its support. We now have a tutorial for automated fluorescence lifetime imaging (FLIM). Over the last couple of months we received more and more questions about automated FLIM. Not everyone realizes that our software package LI-FLIM enables measurement automation via its ActiveX interface. So time resolved photoluminescence is possible with your custom written software or scripts.

This new tutorial is based on use of MATLAB, but it can easily be translated to e.g. C++, Python, Perl, or other programming languages. The tutorial will give you insight in the possibilities of LI-FLIM.

If you have questions about the functions of LI-FLIM, please check the user manual or email support.