Updated Lambert Instruments Website

We have updated the Lambert Instruments website. It has been redesigned and optimized for both desktop computers and mobile devices. 

The product pages have been updated to give a simple overview of the features and specifications of our products. For further information, there are downloadable specifications available for most products. And if you have other questions about a product, you can simply press the green button at the top of each product page to contact us.

All the application notes and technology notes have also been moved to the updated website. We categorized and tagged them, so it's easier to find the information you are looking for. After clicking Applications or Technologies in the navigation bar, you will see an overview of the most recent posts. When you scroll down, you can select a category that describes the field or the product you are interested in. If you want to learn about a specific subject, you can click one of the tags. And if the categories and tags don't list the subject you are looking for, then you can always use the search field:

 

If you have any questions about the updated website, then please let us know.

Mastering Sustained Megahertz Gating

Lambert Instruments announces a brand new megahertz gate unit for its intensified high-speed imaging products. The Lambert Instruments megahertz gate unit is a high-voltage pulse generator that generates up to a million high-voltage pulses per second. These pulses are sent to an image intensifier, enabling high-resolution gated imaging for applications like time-domain fluorescence lifetime imaging and single-photon detection.

The megahertz gate unit is an important step forward in intensifier gating, as it supports continuous operation at 1 MHz. The advanced pulse generator provides optimal image intensifier operation at all repetition rates, even at the peak repetition rate. This high-quality signal further improves the imaging results of Lambert Instruments intensified imaging solutions.

For more information, please contact our sales team.

Lambert Instruments Celebrates the 10th Anniversary of the LIFA

This year marks the 10th anniversary of the LIFA. With the first Lambert Instruments FLIM Attachment (LIFA) a decade ago, we introduced an easy and fast approach to fluorescence lifetime imaging. Since then, we advanced our imaging and analysis software; we improved our hardware and made it more compact; and we added compatibility with third-party hardware. But at the heart of the LIFA experience are still the features that matter most to our users. They are using the LIFA every day, because it is the easiest and fastest system for fluorescence lifetime imaging microscopy.

Read our interview with Dr. Kees Jalink at the Netherlands Cancer Institute about his experiences with the LIFA.

Lambert Instruments Ships First LIFA to India

Lambert Instruments is proud to announce the shipment of the first LIFA to India, following our increased effort to meet the growing demand for scientific imaging equipment in Asia.

Professor Inamdar studies quantum-dot-based FRET at Karnatak University in Dharwad, India. His interest in the LIFA was sparked by its ability to quickly measure fluorescence lifetimes. "We used to do FRET measurements using fluorescence intensities," says professor Inamdar, "but with ratiometric techniques you can't measure fluorescence lifetimes. So we started looking for a FLIM system, because fluorescence lifetimes are very useful for our kind of work."

With the LIFA, Inamdar hopes to advance his research and extend it to biological applications. The LIFA is the ideal solution for such applications, because it can record a lifetime image in a matter of seconds, practically making the process a real-time experience.

Inamdar was supported by Dynotech Instruments during the purchase process. Dynotech Instruments is a leading supplier of products and solutions in the field of optics in India, including Lambert Instruments products.

From 23rd to 25th of September, Lambert Instruments and Dynotech Instruments will be at the Laser World of Photonics 2014 in Bangalore. Visit us at booth B006 in hall 3A for more information about the LIFA and our other products.

Introducing the HiCAM Fluo: An Intensified High-Speed Camera for Fluorescence Applications

Introducing the HiCAM Fluo: A high-speed camera for fluorescence applications. It records high resolution images at a framerate of 540 fps in the most challenging lighting conditions. The HiCAM Fluo is able to capture the dim light of fluorescence by using a cooled image intensifier. Packed into a compact aluminum enclosure, it is easy to attach the HiCAM Fluo to any fluorescence microscope.

Fast

With its 2.9 megapixel (1696 x 1710 pixels) sensor, the HiCAM Fluo can capture 540 frames per second at full resolution. It can achieve even higher framerates at reduced resolutions, up to 5000 fps at 480 x 480 pixels.
 

Single-Photon Sensitive

An image intensifier boosts the intensity of the incoming light to ensure clear images of fluorescence light. The HiCAM Fluo can detect light intensities down to the single-photon level.
 

Cooled

The image intensifier of the HiCAM Fluo is cooled to minimize thermal noise. Our advanced cooling system provides a stable temperature for optimal imaging performance.
 

Streaming

All images are streamed directly to your computer over a high-speed interface, so the total recording time is only limited by the amount of available hard disk space.
 

More information

The official launch of the HiCAM Fluo will be at the Laser World of Photonics in Bangalore, India on September 23. Lambert Instruments and our distributor Dynotech Instruments will be at booth B006.

For detailed technical specifications of the HiCAM Fluo, please visit our HiCAM Fluo page.

Newsletter: How to Fingerprint Oils and Tell Them Apart

Quality control is an important part of any production process. A series of checks on a randomly selected product tells you whether its quality meets the required standards. The TRiCAM is a fast and accurate instrument for quality control.

Taotao Mu at the Beijing Institute of Technology developed a method to quickly determine the fluorescent fingerprint of both motor oils and edible oils. Consistent production quality is critical for these products, making quick and reliable quality control all the more important.

Mu uses a gated TRiCAM and a pulsed laser for his experiments. He studies the decay of the fluorescence light intensity after excitation at several wavelengths. From this data, he compiles a contour diagram of fluorescence intensity: the equivalent of a fluorescent fingerprint of the oil. This allows him to tell apart wide varieties of oils.

Contour diagrams of fluorescence intensities of (a) olive, (b) rapeseed, (c) grapeseed, (d) soybean, (e) corn, (f, g, h) peanut oils. The fluorescence wavelength and detection time after the excitation light pulse are used as axes.

The TRiCAM can record images using gate widths down to less than 3 ns. By carefully synchronizing the laser and the camera, the decay of the fluorescence light intensity can be studied in great detail.

For more information, please refer to our application note about time-resolved fluorescence in the analysis of edible oils.

FLIM-FRET Workshop in Glasgow

Lambert Instruments invites you to register for the FLIM-FRET workshop on September 3 and 4 at the Beatson Institute for Cancer Research in Glasgow. The event is hosted by the Beatson Advanced Imaging Resource and will be suitable for all levels of experience.
 

Program

September 3 will consist of a full program with lectures and hands-on sessions from experts in FLIM and FRET. The second day of the workshop is optional and reserved for attendees who would like a more in-depth fluorescence microscopy training. You are welcome to bring your own samples to the sessions on the second day.
 

Further information

For further information regarding registration and hotels, please refer to the workshop flyer.

Newsletter: Intensify Your Camera

Does your camera struggle to record clear images in low-light situations? You could increase the exposure time to capture more light. But that may introduce motion blur. What you need is a camera with a better light sensitivity. Instead of buying a new camera, you can improve the light sensitivity of your current camera by equipping it with an image intensifier. Upgrading your camera in this way saves you the trouble of implementing a new camera into your setup.

The image below illustrates the effect of the image intensifier. It shows images of a coffee mug that we recorded with and without an image intensifier in a room with little light. The left part of the image was recorded with a camera without an image intensifier. The right part was recorded with the same camera, but with an intensifier coupled to the sensor. The gain of the intensifier was set to a low value, so the light intensity can still be boosted much further.

Note how much brighter the right part of the image is, despite the fact that it was recorded with a shorter exposure time than the left part of the image.

Our new Intensify Your Camera service is the easiest way to boost the light sensitivity of your camera. Based on the specifications of your camera and the optical requirements for your setup, we will make a detailed recommendation for the required image intensifier and optics. You then send your camera to Lambert Instruments. Our skilled technicians will fiber-optically couple the image intensifier to your camera. They have years of experience doing this because we use the same technique for our own camera systems.

The image intensifier increases your camera's light sensitivity by orders of magnitude. And it enables nanosecond exposures when combined with our advanced gating technologies. With such short exposure times motion blur is no longer an issue, not even in low-light situations. And because we attach the image intensifier to your own camera, you can keep using the software and analysis methods you are already familiar with.

Lambert Instruments has over two decades of experience with image intensifiers. We use them in our imaging systems and have equipped many types of sensors with image intensifiers. You can read about coupling an image intensifier to a sensor or if you are interested in adding an image intensifier to your camera, you can submit your camera specifications.

Application note: Time-resolved fluorescence in the analysis of edible oils

Taotao Mu at the Beijing Institute of Technology used a TRiCAM to make contour diagrams of the time-resolved fluorescence intensities of different kinds of edible oils. This method greatly improves the identification capabilities and outperforms traditional fluorescence analysis.

In the application note 'Time-resolved fluorescence in the analysis of edible oils' you can read all about the advantages of the short gating periods the TRiCAM enables.

Application note: Oxygen transport using phosphorescence lifetime imaging

We have published a new application note on the use of phosphorescence lifetime imaging of oxygen transport across slippery and curved gas-liquid interfaces. The note describes the experiments performed by Dr. Elif Karatay during her PhD studies at the University of Twente (NL). She used an oxygen sensitive luminescent dye to image the oxygen concentration in flowing aqueous solutions. The eXtended Lambert Instruments FLIM Attachment (LIFA-X) enabled her to image lifetimes up to 1 ms.

Read more: Oxygen transport using phosphorescence lifetime imaging.

Dr. Karatay is currently doing her postdoctoral research at Stanford University in the United States.

Product release: TRiCATT Time-Resolved intensifier Camera Attachment

Hereby we release the TRiCATT (Time Resolved intensified Camera ATTachment) intensifier. It is in line with the release of the TRiCAM, the intensified CCD camera for scientific applications.

In fact, the TRiCATT is the successor of the II18:

  • The TRiCATT M is the modulated intensifier component for frequency domain FLIM, and is the successor of the II18MD and II18MDS.
  • The TRiCATT G is the gated intensifier for time domain and represents the successor of the II18 G(D)(F).

Like the TRiCAM, the TRiCATT can be offered for scientific applications that require 1) low-light level imaging, 2) ultra-short exposures through fast gating, and/or 3) frequency domain imaging using lock-in detection.

There are three models, the TRiCATT M for frequency domain FLIM, the TRiCATT G for time domain (gated mode) and the TRiCATT GM for both frequency and time domain imaging. For a detailed specification of the features and applications of this versatile product please see here.

Product release: Streaming high-speed ICMOS camera

Lambert Instruments has introduced a streaming version of its HiCAM 500 intensified high-speed CMOS camera that includes a full Camera Link® interface, making it possible to directly stream 500 fps (full frame) to your computer. The streaming HiCAM can be used in a smart camera setup by combining it with an FPGA enabled framegrabber.

The first streaming HiCAM was delivered to Zarm, the center for applied space technology and microgravity of the University of Bremen in Germany. At Zarm the HiCAM is used for a combustion experiment under microgravity conditions. The experiment is prepared in the Zarm drop tower and finally performed as part of an ESA space mission in 2014. A specific mechanical design of the fiber-optic coupling was implemented to cope with the g-forces involved.

The Lambert Instruments HiCAM is a hybrid intensified CMOS camera designed for high-speed imaging and/or ultra-fast gating at low light-levels. The HiCAM is equipped with the latest Gen II and Gen III image intensifiers providing the best possible resolution and sensitivity. Applications in the life sciences include calcium imaging, fluorescence imaging and widefield fluorescence correlation spectroscopy. Applications in the engineering sciences include combustion, particle image velocimetry and laser induced fluorescence.

Change of ownership

Starting from May 2nd 2013, our director Bert van Geest retired from Lambert Instruments BV. The company has been sold to a new owner and will be directed by Mr. Gerard Verheule as CEO. Gerard has a long background in technical commercial activities as owner and director of several wholesale companies in electrotechnical and electronic products. He will continue Lambert Instruments BV in its current state including staff and facilities.

The company will continue to focus on the development, production and worldwide sales of high-end products for time-resolved imaging at low light levels, including intensified High-Speed cameras and FLIM systems, guided by customer feedback. A smooth transition will be assured by the services of Bert van Geest as an advisor.

Product release: TRiCAM Time-Resolved intensified Camera

The TRiCAM (Time Resolved intensified CAMera) intensified CCD is the new compact intensified CCD camera for scientific applications that require

  1. low-light level imaging,
  2. ultra-short exposures through fast gating, and/or
  3. frequency domain imaging using lock-in detection.

With its fast-gating module the TRiCAM offers gating down to 2 ns (FWHM) at 100 kHz.

There are three models, the TRiCAM M for frequency domain FLIM, the TRiCAM G for time domain (gated) imaging and the TRiCAM GM for both frequency and time domain imaging. For a detailed specification of the features and applications of this versatile ICCD please visit the specific product pages for the TRiCAM M and the TRiCAM G.

Mechanical shutter for HiCAM and HiCATT

Being very sensitive tools for ultra high speed imaging the HiCATT and HiCAM are often exposed to high energy radiation. Applications like combustion experiments or UV-spectroscopy in a synchrotron beamline are good examples for situations where the photocathode can be prone to harmful scattered or direct incident light. Although this is fine during the actual measurements, extended exposure to stray light or laser bundles during alignment and other activities may damage the photocathode.

To avoid fiddling with lenscaps between measurements we have added a mechanical shutter to our intensified high speed product line. The shutter is encased in a aluminum housing with a standard F-mount input that replaces the original F-mount adapter of the HiCATT or HiCAM. The back focal distance of the F-mount input is unchanged so any F-mount objective can be used. The shutter comes with a power supply and a remote shutter controller with a timer. The timer can be used to automatically close shutter after a predetermined time. The remote has a stylish and ergonomic design and a large LCD screen. The shutter can also be controlled by a external TTL signal. When purchased together with one of our digital interfaces (s, d or p option) the shutter is controlled by the software of the digital interface.

Tutorial: Automated FLIM

Lambert Instruments keeps improving and expanding its support. We now have a tutorial for automated fluorescence lifetime imaging (FLIM). Over the last couple of months we received more and more questions about automated FLIM. Not everyone realizes that our software package LI-FLIM enables measurement automation via its ActiveX interface. So time resolved photoluminescence is possible with your custom written software or scripts.

This new tutorial is based on use of MATLAB, but it can easily be translated to e.g. C++, Python, Perl, or other programming languages. The tutorial will give you insight in the possibilities of LI-FLIM.

If you have questions about the functions of LI-FLIM, please check the user manual or email support.