Toggel
Toggel
Toggel
Next-generation
FLIM camera
Toggel simplifies FLIM for researchers and imaging centers by combining excellent light sensitivity with easy image acquisition and data analysis.
Applications
Applications
Applications
Live-cell imaging
Track how the lifetimes in your sample change over time with the built-in time-lapse feature. Just set the duration and time between measurements and our software does the rest.
This video shows a time-lapse of HeLa cells. After 150 seconds isoproterenol is added, which results in a rapid increase of cAMP and a corresponding increase in fluorescence lifetime. This is followed by cAMP decomposition and a steady decrease in fluorescence lifetime.
FLIM data courtesy of the Netherlands Cancer Institute.
Single-image FLIM
Demonstration of the Lambert Instruments Toggel camera for single-image FLIM (siFLIM) detection of histamine-induced alterations in Ca2+ concentration. Tiny oscillations in Ca2+ levels (~2.5 s periods) are observed after addition of histamine. Such small and rapid transients would go completely unnoticed when recorded by conventional FLIM.
Video courtesy of the Netherlands Cancer Institute.
Bacteria research
B. subtilis cells where GFP-tagRFP fluorophores are linked are mixed in a 1:1 ratio with B. subtilis cells where the GFP-tagRFP fluorophores are cleaved apart; resulting in a mix of cells with either short GFP fluorescence lifetime due to quenching by tagRFP or long GFP fluorescence lifetime.
Image courtesy of the University of Groningen.
High-throughput screening
Researchers at the University of Amsterdam developed a multi-position fluorescence lifetime imaging (FLIM) screening method to screen for bright FPs. However, this method can be applied to any experiment in which the fluorescence lifetime is an important parameter.
Toggel image gallery
The images below were recorded with the Toggel and processed in the LIFA software that ships with the Toggel.
Stitched lifetime images were stitched in ImageJ.
Specifications
Specifications
Specifications
Specifications are subject to change without prior notice.
Camera
Up to 30 fps (dual images)
13 kHz - 80 MHz modulation frequencies
Camera Link computer interface
Sensor temperature stabilized at 20 C
Image sensor
Proprietary CCD sensor
504 x 512 pixels
24 x 24 um pixel size
14 bit pixel bit depth
67 000 e- full-well capacity
25 e- readout noise
2 000 : 1 (66 dB) dynamic range
400 e-/s/pixel (at 20 C) dark current
50% fill factor
Image sensor
Toggel features a unique image sensor that combines excellent light sensitivity with advanced fluorescence lifetime imaging
capabilities. This image sensor was designed and optimized specifically for fluorescence lifetime imaging applications and enables lifetime imaging at unprecedented frame rates with the single-image fluorescence lifetime imaging microscopy (siFLIM) method.
Publications
Publications
User publications
Researchers around the world use Lambert Instruments products in their studies. Below is an overview of the most recent publications describing research that was done using a Toggel. For a complete overview of applications, please refer to our applications pages.
