Demonstration of the Lambert Instruments Toggel camera for single-image FLIM (siFLIM) detection of histamine-induced alterations in Ca2+ concentration. Tiny oscillations in Ca2+ levels (~2.5 s periods) are observed after addition of histamine. Such small and rapid transients would go completely unnoticed when recorded by conventional FLIM.
Video courtesy of the Netherlands Cancer Institute.
The images below were recorded with the Toggel and processed in the LIFA software that ships with the Toggel.
Stitched lifetime images were stitched in ImageJ.
Specifications are subject to change without prior notice.
Up to 30 fps (dual images)
13 kHz - 80 MHz modulation frequencies
Camera Link computer interface
Sensor temperature stabilized at 20 C
Proprietary CCD sensor
504 x 512 pixels
24 x 24 um pixel size
14 bit pixel bit depth
67 000 e- full-well capacity
25 e- readout noise
2 000 : 1 (66 dB) dynamic range
400 e-/s/pixel (at 20 C) dark current
50% fill factor
Researchers around the world use Lambert Instruments products in their studies. Below is an overview of the most recent publications describing research that was done using a Toggel. For a complete overview of applications, please refer to our applications pages.