Toggel


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Toggel


Toggel

Next-generation
FLIM camera

Toggel simplifies FLIM for researchers and imaging centers by combining excellent light sensitivity with easy image acquisition and data analysis.

 
 Toggel is a next-generation fluorescence lifetime imaging microscopy (FLIM) camera based on a modulated CCD image sensor
 

Applications


Applications


Applications

 

Live-cell imaging

Track how the lifetimes in your sample change over time with the built-in time-lapse feature. Just set the duration and time between measurements and our software does the rest.

This video shows a time-lapse of HeLa cells. After 150 seconds isoproterenol is added, which results in a rapid increase of cAMP and a corresponding increase in fluorescence lifetime. This is followed by cAMP decomposition and a steady decrease in fluorescence lifetime.

FLIM data courtesy of the Netherlands Cancer Institute.

 
 

Single-image FLIM

Demonstration of the Lambert Instruments Toggel camera for single-image FLIM (siFLIM) detection of histamine-induced alterations in Ca2+ concentration. Tiny oscillations in Ca2+ levels (~2.5 s periods) are observed after addition of histamine. Such small and rapid transients would go completely unnoticed when recorded by conventional FLIM.

Video courtesy of the Netherlands Cancer Institute.

 
 

Bacteria research

B. subtilis cells where GFP-tagRFP fluorophores are linked are mixed in a 1:1 ratio with B. subtilis cells where the GFP-tagRFP fluorophores are cleaved apart; resulting in a mix of cells with either short GFP fluorescence lifetime due to quenching by tagRFP or long GFP fluorescence lifetime.

Image courtesy of the University of Groningen.

 
 

High-throughput screening

Researchers at the University of Amsterdam developed a multi-position fluorescence lifetime imaging (FLIM) screening method to screen for bright FPs. However, this method can be applied to any experiment in which the fluorescence lifetime is an important parameter.

 
 

Toggel image gallery

The images below were recorded with the Toggel and processed in the LIFA software that ships with the Toggel.
Stitched lifetime images were stitched in ImageJ.

After 150 seconds isoproterenol is added, which results in a rapid increase of cAMP and a corresponding increase in fluorescence lifetime. This is followed by cAMP decomposition and a steady decrease in fluorescence lifetime. FLIM data courtesy of the Netherlands Cancer Institute.
Fluorescence lifetime of chlorophyll upon excitation with a 488 nm LED imaged with the Lambert Instruments Toggel FLIM camera. The fluorescence lifetime is presented as a pseudo-color overlay on the image. Interval between FLIM image acquisitions was 5 seconds and total duration of the time-lapse was 5 minutes.
Demonstration of the Lambert Instruments Toggel camera for single-image FLIM (siFLIM) detection of histamine-induced alterations in Ca2+ concentration. Tiny oscillations in Ca2+ levels (~2.5 s periods) are observed after addition of histamine. Such small and rapid transients would go completely unnoticed when recorded by conventional FLIM.

Specifications


Specifications


Specifications

Specifications are subject to change without prior notice.

 

Camera

 

 

 

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Up to 30 fps (dual images)

13 kHz - 80 MHz modulation frequencies

Camera Link computer interface

Sensor temperature stabilized at 20 C


Image sensor

 

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Proprietary CCD sensor

504 x 512 pixels

24 x 24 um pixel size

14 bit pixel bit depth

67 000 e- full-well capacity

25 e- readout noise

2 000 : 1 (66 dB) dynamic range

400 e-/s/pixel (at 20 C) dark current

50% fill factor


Image sensor

Toggel features a unique image sensor that combines excellent light sensitivity with advanced fluorescence lifetime imaging
capabilities. This image sensor was designed and optimized specifically for fluorescence lifetime imaging applications and enables lifetime imaging at unprecedented frame rates with the single-image fluorescence lifetime imaging microscopy (siFLIM) method.