Live-cell imaging

Track how the lifetimes in your sample change over time with the built-in time-lapse feature. Just set the duration and time between measurements and our software does the rest.

This video shows a time-lapse of HeLa cells. After 150 seconds isoproterenol is added, which results in a rapid increase of cAMP and a corresponding increase in fluorescence lifetime. This is followed by cAMP decomposition and a steady decrease in fluorescence lifetime.

FLIM data courtesy of the Netherlands Cancer Institute.

Single-image FLIM

Demonstration of the Lambert Instruments Toggel camera for single-image FLIM (siFLIM) detection of histamine-induced alterations in Ca2+ concentration. Tiny oscillations in Ca2+ levels (~2.5 s periods) are observed after addition of histamine. Such small and rapid transients would go completely unnoticed when recorded by conventional FLIM.

Video courtesy of the Netherlands Cancer Institute.

Bacteria research

B. subtilis cells where GFP-tagRFP fluorophores are linked are mixed in a 1:1 ratio with B. subtilis cells where the GFP-tagRFP fluorophores are cleaved apart; resulting in a mix of cells with either short GFP fluorescence lifetime due to quenching by tagRFP or long GFP fluorescence lifetime.

Image courtesy of the University of Groningen.

High-throughput screening

Researchers at the University of Amsterdam developed a multi-position fluorescence lifetime imaging (FLIM) screening method to screen for bright FPs. However, this method can be applied to any experiment in which the fluorescence lifetime is an important parameter.

Widefield

On widefield microscopes, the Toggel camera in combination with the Multi-LED offers a capable yet compact FLIM solution. The Toggel is compatible with the camera port of widefield microscopes and the Multi-LED is compatible with the standard epifluorescence port of widefield microscopes.

Spinning-disk confocal

Being a camera-based system, the Lambert Instruments LIFA system for frequency-domain FLIM is compatible with multi-beam confocal microscopy techniques, most notably the Yokogawa CSU spinning disk series (based on the Nipkow disk scanner), and the VTInfinity series by Visitech International.

TIRF

Total Internal Reflection Fluorescence (TIRF) microscopy facilitates extremely high-contrast visualization and thereby high sensitivity of fluorescence near the cover glass. Typically, the optical section adjacent to the cover glass is about 100 nm. The unique combination of TIRF and frequency-domain FLIM makes it possible to measure lifetimes of, for instance, small focal adhesions near the cover glass.

Camera

Up to 30 fps (dual images)

13 kHz - 80 MHz modulation frequencies

Camera Link computer interface

Sensor temperature stabilized at 20 C

Image sensor

Proprietary CCD sensor

504 x 512 pixels

24 x 24 um pixel size

14 bit pixel bit depth

67 000 e- full-well capacity

< 35 e- readout noise

2 000 : 1 (66 dB) dynamic range

400 e-/s/pixel (at 20 C) dark current

50% fill factor

User publications

Researchers around the world use Lambert Instruments products in their studies. Below is an overview of the most recent publications describing research that was done using a Toggel. For a complete overview of applications, please refer to our applications pages.

Featured Toggel users