Toggel
Toggel
Toggel
A next-generation fluorescence lifetime imaging microscopy (FLIM) camera. Toggel simplifies FLIM for researchers and imaging centers by combining excellent light sensitivity with easy image acquisition and data analysis.
Applications
Applications
Applications
Single-Image FLIM
Demonstration of the Lambert Instruments Toggel camera for single-image FLIM (siFLIM) detection of histamine-induced alterations in Ca2+ concentration. Tiny oscillations in Ca2+ levels (~2.5 s periods) are observed after addition of histamine. Such small and rapid transients would go completely unnoticed when recorded by conventional FLIM.
Video courtesy of the Netherlands Cancer Institute.
Live-Cell Imaging
Track how the lifetimes in your sample change over time with the built-in time-lapse feature. Just set the duration and time between measurements and our software does the rest.
Time-lapse of EPAC sensor courtesy of Netherlands Cancer Institute .
Bacteria Research
B. subtilis cells where GFP-tagRFP fluorophores are linked are mixed in a 1:1 ratio with B. subtilis cells where the GFP-tagRFP fluorophores are cleaved apart; resulting in a mix of cells with either short GFP fluorescence lifetime due to quenching by tagRFP or long GFP fluorescence lifetime. (Image courtesy of University of Groningen)
High-Throughput Screening
Researchers at the University of Amsterdam developed a multi-position fluorescence lifetime imaging (FLIM) screening method to screen for bright FPs. However, this method can be applied to any experiment in which the fluorescence lifetime is an important parameter.
Toggel image gallery
The images below were recorded with the Toggel and processed in the LIFA software that ships with the Toggel.
Specifications
Specifications
Specifications
30 fps
In siFLIM Mode
Up to 80 MHz
Modulation Frequency
24 x 24 µm
Pixel size
Image Sensor
Toggel features a unique image sensor that combines excellent light sensitivity with advanced fluorescence lifetime imaging
capabilities. This image sensor was designed and optimized specifically for fluorescence lifetime imaging applications and enables lifetime imaging at unprecedented frame rates with the single-image fluorescence lifetime imaging microscopy (siFLIM) method.
Parameter
Pixel resolution
Pixel size
Pixel bit depth
Full-well capacity
Readout noise
Dynamic range
Dark current
Sensor type
Fill factor
Frame rate
Modulation frequencies
Sensor cooling
Camera interface
Typical value
504 x 512 pixels
24 x 24 um
14 bit
67 000 e-
25 e-
2 000 : 1 (66 dB)
400 e-/s/pixel (at 20 C)
Proprietary CCD sensor
50%
Up to 30 fps (dual images)
13 kHz - 80 MHz
Stabilized at 20 C
Camera Link
Specifications are subject to change without prior notice.
Publications
Publications
User Publications
Researchers around the world use Lambert Instruments products in their studies. Below is an overview of the most recent publications describing research that was done using a Toggel. For a complete overview of applications, please refer to our applications pages.
